Glycosylation is a major post-translational protein modification known to affect protein folding, stability, activity, molecular interaction, and functionality. N- and O-linked glycosylations represent the most common types of glycosylation. In mass spectrometry, the ionization of glycopeptides is less efficient compared to nonglycosylated peptides, leading to signal suppression and poor detection of glycopeptides. Therefore, enrichment of glycopeptides prior to mass spectrometry is often required.
The Preciex? glycopeptide enrichment Stage-tip uses a proprietary ZIK Glycocapture Resin to capture high-mannose, hybrid, and complex glycosylated peptides from complex protein mixtures for effective downstream analysis by MALDI and ESI-MS. In combination with the included optimized buffers, the ZIK Glycocapture Resin is highly selective for glycosylated peptides and enriches samples without bias towards or loss of particular glycan structures. The enrichment protocol is optimized for efficient removal of non-glycosylated peptides with close to 100% glycopeptide recovery (as quantitatively assessed by N-Glycan mapping) and offers the flexibility to perform both site-specific determination of the glycan structures and parallel analysis of the attached peptides.
Process of use: centrifugation, vacuum, positive pressure.
Formats: 10μL and 200μL.
For any other offline or online format, contact us.
*200μL tips are available for different Liquid Handling Automation systems (Zymark, Multiprobe, Tecan, Hamilton, Gilson, Qiagen, and many more) without any further adaptor needed.
Features of Glycopeptide Stage-tip:
? Higher selectivity for glycopeptides
? Achieve ready-to-measure glycopeptides
? Work efficiently to N-, O-linked and glycosylphosphatidylinositol-anchored glycopeptides
? Suitable for manual and automated processing
Common product Specifications:
Sample | Glycopeptide |
Chromatographic bed | HILIC resin |
Volume | 200μL |
Capacity | 2ug |
pH Range | 2-12 |
Temperature | 4-60°C |
Quantity | 96 Tips |
Detection Method | HPLC, Mass spectrometry, Capillary electrophoresis, Atomic absorption spectroscopy |
Ordering Information:
Description | Format | Capacity | Part Number |
Preciex Glycopeptide | 10μL | 0.5μg | 2109001 |
Preciex Glycopeptide | 200μL | 2μg | 2109022 |
Preciex Glycopeptide | 200μL | 10μg | 2109103 |
User Guide
http://freshbioscience.com/public/upload/202210/20221008140603.pdf
Material Safety Data Sheets (MSDS)
